Yeast DNA Polymerase η Utilizes an Induced-Fit Mechanism of Nucleotide Incorporation

@article{Washington2001YeastDP,
  title={Yeast DNA Polymerase η Utilizes an Induced-Fit Mechanism of Nucleotide Incorporation},
  author={M. Todd Washington and L. T. Om Prakash and Satya Prakash},
  journal={Cell},
  year={2001},
  volume={107},
  pages={917-927}
}
DNA polymerase eta (Poleta) is unique among eukaryotic DNA polymerases in its proficient ability to replicate through distorting DNA lesions, and Poleta synthesizes DNA with a low fidelity. Here, we use pre-steady-state kinetics to investigate the mechanism of nucleotide incorporation by Poleta and show that it utilizes an induced-fit mechanism to selectively incorporate the correct nucleotide. Poleta discriminates poorly between the correct and incorrect nucleotide at both the initial… CONTINUE READING

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