Yeast/E. coli shuttle vectors with multiple unique restriction sites

@article{Hill1986YeastECS,
  title={Yeast/E. coli shuttle vectors with multiple unique restriction sites},
  author={John E. Hill and Alan M. Myers and T. J. Koerner and Alexander Tzagoloff},
  journal={Yeast},
  year={1986},
  volume={2}
}
Key ResultTwo yeast/E. coli shuttle vectors have been constructed.
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomyces cerevisiae
TLDR
This work has constructed a set of 42 plasmid shuttle vectors based on the widely used pRS series for use in the budding yeast Saccharomyces cerevisiae and the bacterium Escherichia coli, and determined several errors in existing publicly available sequences for several commonly used yeast plasmids.
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
TLDR
A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae to perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
A family of low and high copy replicative, integrative and single‐stranded S. cerevisiae/E. coli shuttle vectors
We describe a set of replicative, integrative and single‐stranded shuttle vectors constructed from the pUC19 plasmid that we use routinely in our experiments. They bear a yeast selectable marker:
A shuttle vector series for precise genetic engineering of Saccharomyces cerevisiae
TLDR
The pRG shuttle vector series is constructed, consisting of single‐ and multi‐copy integrative, centromeric and episomal plasmids with marker genes for the selection in all commonly used auxotrophic yeast strains, enabling the efficient cloning of genes and their maintenance in yeast cells with improved copy number control.
Screening and identification of yeast sequences that cause growth inhibition when overexpressed
TLDR
A galactose-inducible expression library with partially digested Saccharomyces cerevisiae genomic DNA fragments inserted downstream of the GAL10 promoter indicated that the inserts encoded part or all of the URA2, RBP1, TPK3, SAC7, BOI1, and BNI1 genes, and also open reading frames from chromosomes IV, V, IX, XI, and XIII.
A family of vectors that facilitate transposon and insertional mutagenesis of cloned genes in yeast
TLDR
Two sets of plasmid vectors that facilitate the identification of regions of complementation in cloned genomic inserts via transposon or insertional mutagenesis and gene disruption procedures performed in vitro are described.
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