Y-family DNA polymerases in mammalian cells

  title={Y-family DNA polymerases in mammalian cells},
  author={Caixia Guo and J. Nicole Kosarek-Stancel and Tie-Shan Tang and Errol C. Friedberg},
  journal={Cellular and Molecular Life Sciences},
Eukaryotic genomes are replicated with high fidelity to assure the faithful transmission of genetic information from one generation to the next. The accuracy of replication relies heavily on the ability of replicative DNA polymerases to efficiently select correct nucleotides for the polymerization reaction and, using their intrinsic exonuclease activities, to excise mistakenly incorporated nucleotides. Cells also possess a variety of specialized DNA polymerases that, by a process called… 

Regulation of Translesion DNA Synthesis in Mammalian Cells.

Current understandings of regulation of TLS process at the levels of protein-protein interactions, post-translational modifications as well as transcription and non-coding RNAs are summarized.

Role of DNA polymerase κ in the maintenance of genomic stability

How DNA polymerase kappa (Pol κ), one of the most highly conserved TLS DNA polymerases, is involved in each of these pathways and thereby coordinates them to choreograph the response to a stalled replication fork is described.

Structure of human DNA polymerase iota and the mechanism of DNA synthesis

The structural peculiarities of the Pol ι active site are considered and possible mechanisms that ensure the unique behavior of the enzyme on damaged and undamaged DNA are discussed.

Translesion DNA polymerases in eukaryotes: what makes them tick?

Although most of the currently identified eukaryotic DNA polymerases have been implicated in TLS, the best characterized are those belonging to the “Y-family” of DNA polymerase (pols η, ι, κ and Rev1), which are thought to play major roles in the TLS of persisting DNA lesions in coordination with the B-family polymerase, pol ζ.

Structural basis of ubiquitin recognition by translesion synthesis DNA polymerase ι.

Using NMR spectroscopy, the structure of a complex of human Polι UBM2 and Ubiquitin is determined, revealing a novel ubiquitin recognition fold consisting of two α-helices separated by a central trans-proline residue conserved in all UBMs.

Structural model of the Y-Family DNA polymerase V/RecA mutasome.




Damage repair DNA polymerases Y.

  • Wei Yang
  • Biology
    Current opinion in structural biology
  • 2003

Ubiquitination of PCNA and the Polymerase Switch in Human Cells

The possible signals that might trigger ubiquitination of PCNA, whether PCNA becomes de-ubiquitinated after TLS has been accomplished and the role of the hREV1 protein in TLS are discussed.

Proficient and Accurate Bypass of Persistent DNA Lesions by DinB DNA Polymerases

It is suggested that some Y-family polymerases, including DinB and pol κ, bypass certain types of DNA damage with greater proficiency than an undamaged template and do so relatively accurately.

Localisation of human DNA polymerase kappa to replication foci.

Polkappa is mostly localised uniformly in the nucleus of undamaged cells, but could be also concentrated in PCNA-containing replication foci, and Mutagenesis experiments indicated that Polkappa involvement may affect the accuracy of DNA replication.

Translesion synthesis: Y-family polymerases and the polymerase switch.

Proliferating Cell Nuclear Antigen-dependent Coordination of the Biological Functions of Human DNA Polymerase ι*

It is reported here that an interaction between human DNA polymerase ι (polι) and the proliferating cell nuclear antigen (PCNA) stimulates the processivity of polι in a template-dependent manner in vitro.

DNA polymerase iota and related rad30-like enzymes.

Based upon in vitro studies, it appears that Pol iota has the lowest fidelity of any eukaryotic polymerase studied to date and the authors speculate as to the possible cellular functions of such a remarkably error-prone DNA polymerase.

Physical and functional interactions of human DNA polymerase eta with PCNA.

Evidence is provided for the physical interaction of hPoleta with proliferating cell nuclear antigen (PCNA) and show that mutations in the PCNA binding motif of h Poleta inactivate this interaction.

Eukaryotic translesion synthesis DNA polymerases: specificity of structure and function.

This review focuses on eukaryotic translesion synthesis (TLS) DNA polymerases, and the emphasis is on Saccharomyces cerevisiae and human Y-family polymerases (Pols) eta, iota, kappa, and Rev1, as