Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis.

Abstract

Most eukaryotic cells elaborate several proteoglycans critical for transmitting biochemical signals into and between cells. However, the regulation of proteoglycan biosynthesis is not completely understood. We show that the atypical secretory kinase family with sequence similarity 20, member B (Fam20B) phosphorylates the initiating xylose residue in the proteoglycan tetrasaccharide linkage region, and that this event functions as a molecular switch to regulate subsequent glycosaminoglycan assembly. Proteoglycans from FAM20B knockout cells contain a truncated tetrasaccharide linkage region consisting of a disaccharide capped with sialic acid (Siaα2-3Galβ1-4Xylβ1) that cannot be further elongated. We also show that the activity of galactosyl transferase II (GalT-II, B3GalT6), a key enzyme in the biosynthesis of the tetrasaccharide linkage region, is dramatically increased by Fam20B-dependent xylose phosphorylation. Inactivating mutations in the GALT-II gene (B3GALT6) associated with Ehlers-Danlos syndrome cause proteoglycan maturation defects similar to FAM20B deletion. Collectively, our findings suggest that GalT-II function is impaired by loss of Fam20B-dependent xylose phosphorylation and reveal a previously unappreciated mechanism for regulation of proteoglycan biosynthesis.

DOI: 10.1073/pnas.1417993111

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@article{Wen2014XylosePF, title={Xylose phosphorylation functions as a molecular switch to regulate proteoglycan biosynthesis.}, author={Jianzhong Wen and Junyu Xiao and Meghdad Rahdar and Biswa Pronab Choudhury and Jixin Cui and Gregory S. Taylor and Jeffrey D Esko and Jack E. Dixon}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2014}, volume={111 44}, pages={15723-8} }