Xylitol production by recombinant Corynebacterium glutamicum under oxygen deprivation

@article{Sasaki2009XylitolPB,
  title={Xylitol production by recombinant Corynebacterium glutamicum under oxygen deprivation},
  author={Miho Sasaki and Toru Jojima and Masayuki Inui and Hideaki Yukawa},
  journal={Applied Microbiology and Biotechnology},
  year={2009},
  volume={86},
  pages={1057-1066}
}
Wild-type Corynebacterium glutamicum produced 0.6 g l−1 xylitol from xylose at a productivity of 0.01 g l−1 h−1 under oxygen deprivation. To increase this productivity, the pentose transporter gene (araE) from C. glutamicum ATCC31831 was integrated into the C. glutamicum R chromosome. Consequent disruption of its lactate dehydrogenase gene (ldhA), and expression of single-site mutant xylose reductase from Candida tenuis (CtXR (K274R)) resulted in recombinant C. glutamicum strain CtXR4 that… CONTINUE READING
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