author={John B. Wallingford and Karen J. Liu and Yixian Zheng},
  journal={Current Biology},
  • J. Wallingford, Karen J. Liu, Yixian Zheng
  • Published 2010
  • Medicine
  • Current Biology
Who or what is Xenopus? Xenopus (literally ‘strange foot’) is a genus of aquatic frogs that are native to southern Africa. These frogs are remarkably tolerant to starvation, disease, and other insults, allowing the genus to span more than 45 degrees of latitude in Sub-Saharan Africa. This formidable constitution also makes them very easy to keep in captivity, and thus Xenopus frogs are very widely used as model organisms for biomedical research (Figure 1). Each Xenopus female lays thousands of… Expand
Xenopus as a platform for discovery of genes relevant to human disease.
This review provides numerous examples describing the successful use of Xenopus to investigate the contribution of patient mutations to complex phenotypes including congenital heart disease and heterotaxy and describes a variety of unique methods that allow us to rapidly recapitulate patients' phenotypes in frogs. Expand
Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis
It is shown that the Tol2 transposon can be remobilized in the genome of X. tropicalis and passed through the germline via a simple breeding strategy of crossing transposase expressing and transpos on lines. Expand
GDF 3 , a BMP inhibitor , regulates cell fate in stem cells and early embryos
INTRODUCTION To begin to understand the molecular character of totipotent cells and the mechanisms of the primary cell fate decisions of the early mammalian embryo, we previously performed a globalExpand
Protein localization screening in vivo reveals novel regulators of multiciliated cell development and function
The subcellular localization in MCCs of ∼200 proteins not previously implicated in cilia biology are defined, and functional analyses arising from the screen provide novel links between actin cytoskeleton and MCC ciliogenesis. Expand
High-content protein localization screening in vivo reveals novel regulators of multiciliated cell development and function
Functional analyses that arose from results of the screen provide novel insights into the mechanisms by which the actin cytoskeleton simultaneously influences diverse aspects of MCC biology, including basal body docking, and ciliogenesis. Expand
Toward the understanding of biology of oocyte life cycle in Xenopus Laevis: No oocytes left behind
This work focuses on the developmental and reproductive biology of the female gametes, oocytes, and eggs, of the African clawed frog Xenopus laevis. Expand
Developmental changes in head movement kinematics during swimming in Xenopus laevis tadpoles
Examination of head movements and swimming kinematics of Xenopus laevis tadpoles with a body length of 10–45 mm and comparison with fictive swimming revealed very similar swimming frequencies for similarly sized animals, including a comparable developmental decrease of the swimming frequency. Expand
Egg-in-Cube: Design and Fabrication of a Novel Artificial Eggshell with Functionalized Surface
A novel artificial eggshell with functionalized surface is proposed, which allows the total amount of oxygen to pass into the egg for the chick embryo culturing and has high observability and accessibility for embryo manipulation. Expand
Quantifying Demyelination in NK venom treated nerve using its electric circuit model
Reduction of myelin in peripheral nerve causes critical demyelinating diseases such as chronic inflammatory demyelinating polyneuropathy, Guillain-Barre syndrome, etc. Clinical monitoring of theseExpand


An in vivo chemical library screen in Xenopus tadpoles reveals novel pathways involved in angiogenesis and lymphangiogenesis.
The present chemical library screening strategy in Xenopus tadpoles represents a rapid and highly efficient approach to identify novel pathways involved in (lymph)angiogenesis. Expand
Direct action of gonadotropin in brain integrates behavioral and reproductive functions
A CNS-mediated role of gonadotropins in a reproductive behavior: the courtship songs of the South African clawed frog, Xenopus laevis is demonstrated, demonstrating a neural role for Gonadotropin in the control of calling behavior, potentially mediated via LHRs in forebrain vocal nuclei. Expand
Ectodermal Factor Restricts Mesoderm Differentiation by Inhibiting p53
It is reported that the Zn-finger protein XFDL156 is an ectodermal, zygotic factor that suppresses mesodermal differentiation and interacts with the C-terminal regulatory region of p53, thereby inhibiting p53 target gene induction and mesodmal differentiation. Expand
A positive feedback mechanism governs the polarity and motion of motile cilia
This work shows that cilia acquire polarity through a sequence of events, beginning with a polar bias set by tissue patterning, followed by a refinement phase, and indicates that during refinement, fluid flow is both necessary and sufficient in determining cilia polarity. Expand
Chromatin Shapes the Mitotic Spindle
The results show that chromatin defines spindle shape and orientation. Expand
Checkpoint signaling from a single DNA interstrand crosslink.
This work demonstrates that ICLs trigger a checkpoint response independently of origin-initiated DNA replication and uncoupling of DNA polymerase and DNA helicase, and suggests that cell sensitivity to crosslinking agents results from both checkpoint and DNA repair defects. Expand
Mechanism of Replication-Coupled DNA Interstrand Crosslink Repair
This work aims to demonstrate the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds. Expand