Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges

@article{Lei2007XenofreeDA,
  title={Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges},
  author={T. Lei and S. Jacob and Imen Ajil-Zaraa and J. Dubuisson and O. Irion and M. Jaconi and A. Feki},
  journal={Cell Research},
  year={2007},
  volume={17},
  pages={682-688}
}
  • T. Lei, S. Jacob, +4 authors A. Feki
  • Published 2007
  • Biology, Medicine
  • Cell Research
    • Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells… CONTINUE READING
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    References

    SHOWING 1-10 OF 67 REFERENCES
    The derivation of clinical‐grade human embryonic stem cell lines
    • 54
    Derivation of a Xeno‐Free Human Embryonic Stem Cell Line
    • 184
    An Autogeneic Feeder Cell System That Efficiently Supports Growth of Undifferentiated Human Embryonic Stem Cells
    • 225
    Variations in humanized and defined culture conditions supporting derivation of new human embryonic stem cell lines.
    • 59
    Human embryonic stem cell lines are contaminated: what should we do?
    • 9
    • PDF
    A Method for the Selection of Human Embryonic Stem Cell Sublines with High Replating Efficiency After Single‐Cell Dissociation
    • 100
    Setting Standards for Human Embryonic Stem Cells
    • 304
    Derivation of human embryonic stem cells in defined conditions
    • 1,030
    • PDF
    The development of 'feeder' cells for the preparation of clinical grade hES cell lines: challenges and solutions.
    • 75
    Derivation and Growing Human Embryonic Stem Cells on Feeders Derived from Themselves
    • 79