Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges

@article{Lei2007XenofreeDA,
  title={Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges},
  author={T. Lei and S. Jacob and Imen Ajil-Zaraa and J. Dubuisson and O. Irion and M. Jaconi and A. Feki},
  journal={Cell Research},
  year={2007},
  volume={17},
  pages={682-688}
}
  • T. Lei, S. Jacob, +4 authors A. Feki
  • Published 2007
  • Biology, Medicine
  • Cell Research
  • Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells… CONTINUE READING
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