XcmI site-containing vector for direct cloning and in vitro transcription of PCR product

@article{ArashiHeese1999XcmISV,
  title={XcmI site-containing vector for direct cloning and in vitro transcription of PCR product},
  author={N Arashi-Heese and Masami Miwa and Hideki Shibata},
  journal={Molecular Biotechnology},
  year={1999},
  volume={12},
  pages={281-283}
}
For TA cloning and the direct in vitro transcription of reverse transcriptase-polymerase chain reaction (RT-PCR) product, we constructed a novel T-vector, pGEMTA.miwa, derived from pGEM-3Zf(+). The vector were designed to produce single thymidine (T) overhangs when digested with a restriction enzyme XcmI. In a useful modification of the direct TA cloning system, the novel T vector can be applied to the direct in vitro transcription of riboprobe from the cloned sequences. The vector has the… CONTINUE READING

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