Xanthine phosphoribosyltransferase from Leishmania donovani. Molecular cloning, biochemical characterization, and genetic analysis.

@article{Jardim1999XanthinePF,
  title={Xanthine phosphoribosyltransferase from Leishmania donovani. Molecular cloning, biochemical characterization, and genetic analysis.},
  author={Armando Jardim and Susan E. Bergeson and Shin-Ru Shih and N. P. Carter and Robert W Lucas and G. Anita Merlin and Peter J. Myler and Kenneth Stuart and Buddy Ullman},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 48},
  pages={34403-10}
}
Xanthine phosphoribosyltransferase (XPRT) from Leishmania donovani is a unique enzyme that lacks a mammalian counterpart and is, therefore, a potential target for antiparasitic therapy. To investigate the enzyme at the molecular and biochemical level, a cDNA encoding the L. donovani XPRT was isolated by functional complementation of a purine auxotroph of Escherichia coli that also harbors deficiencies in the prokaryotic phosphoribosyltransferase (PRT) activities. The cDNA was then used to… CONTINUE READING
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