X-ray structure of cyclodextrin glycosyltransferase complexed with acarbose. Implications for the catalytic mechanism of glycosidases.

Abstract

Crystals of cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans strain 251 were soaked in buffer solutions containing the pseudotetrasaccharide acarbose, a strong amylase- and CGTase inhibitor. The X-ray structure of the complex was elucidated at 2.5-A resolution with a final crystallographic R value of 15.8% for all data between 8.0 and 2.5 A… (More)

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@article{Strokopytov1995XraySO, title={X-ray structure of cyclodextrin glycosyltransferase complexed with acarbose. Implications for the catalytic mechanism of glycosidases.}, author={Boris V Strokopytov and Dirk Penninga and Henri{\"{e}tte J. Rozeboom and Kor H. Kalk and Lubbert Dijkhuizen and Bauke W Dijkstra}, journal={Biochemistry}, year={1995}, volume={34 7}, pages={2234-40} }