X-ray-induced debromination of nucleic acids at the Br K absorption edge and implications for MAD phasing.

@article{Ennifar2002XrayinducedDO,
  title={X-ray-induced debromination of nucleic acids at the Br K absorption edge and implications for MAD phasing.},
  author={Eric Ennifar and Philippe Carpentier and Jean Luc Ferrer and Philippe Walter and Philippe Dumas},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2002},
  volume={58 Pt 8},
  pages={1262-8}
}
Multi-wavelength anomalous dispersion (MAD) using brominated derivatives is considered a common and convenient technique for solving chemically synthesized nucleic acid structures. Here, it is shown that a relatively moderate X-ray dose (of the order of 5 x 10(15) photons mm(-2)) can induce sufficient debromination to prevent structure determination. The decrease in bromine occupancy with radiation dose can be accounted for by a simple exponential, with an estimated rate constant at the… CONTINUE READING

From This Paper

Topics from this paper.
17 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 17 extracted citations

Similar Papers

Loading similar papers…