X-ray crystal structure of michaelis complex of aldoxime dehydratase.

@article{Sawai2009XrayCS,
  title={X-ray crystal structure of michaelis complex of aldoxime dehydratase.},
  author={Hitomi Sawai and Hiroshi Sugimoto and Yasuo Kato and Yasuhisa Asano and Yoshitsugu Shiro and Shigetoshi Aono},
  journal={The Journal of biological chemistry},
  year={2009},
  volume={284 46},
  pages={32089-96}
}
Aldoxime dehydratase (Oxd) catalyzes the dehydration of aldoximes (R-CH=N-OH) to their corresponding nitrile (R-C triple bond N). Oxd is a heme-containing enzyme that catalyzes the dehydration reaction as its physiological function. We have determined the first two structures of Oxd: the substrate-free OxdRE at 1.8 A resolution and the n-butyraldoxime- and propionaldoxime-bound OxdREs at 1.8 and 1.6 A resolutions, respectively. Unlike other heme enzymes, the organic substrate is directly bound… CONTINUE READING