X-ray absorption fine structure determination of pH-dependent U-bacterial cell wall interactions

  title={X-ray absorption fine structure determination of pH-dependent U-bacterial cell wall interactions},
  author={Shelly D. Kelly and Kenneth M. Kemner and Jeremy B. Fein and David A Fowle and Maxim I. Boyanov and Bruce A. Bunker and Nathan W. Yee},
  journal={Geochimica et Cosmochimica Acta},

Zinc sorption to three gram-negative bacteria: combined titration, modeling, and EXAFS study.

The results suggest a dominant role of extracellular polymeric substances in Zn retention processes, although Zn binding to inner cell components cannot be excluded.

Determination of Cu Environments in the Cyanobacterium Anabaena flos-aquae by X-Ray Absorption Spectroscopy

X-ray absorption spectra measured at the Cu K-edge were used to determine the oxidation states and chemical environments of Cu species in the whole-cell and peptidoglycan samples and suggested that Cu(I) was coordinated by amine and thiol ligands.

Coordination of uranium(VI) with functional groups of bacterial lipopolysaccharide studied by EXAFS and FT-IR spectroscopy.

The complexation of uranyl ions with lipopolysaccharide (LPS), the main component of the cell wall of Gram-negative bacteria, was investigated on a molecular level withEXAFS and attenuated total reflection Fourier transform infrared (ATR-FT-IR) spectroscopy over a wide pH range.



Characterizing Heterogeneous Bacterial Surface Functional Groups Using Discrete Affinity Spectra for Proton Binding

A quantitative investigation of the types and densities of proton binding sites on a bacterial surface, Bacillus subtilis, from replicate acid−base titrations on bacteria at two ionic strengths indicated less variability than has been suggested for bacterial surfaces.

Sites of metal deposition in the cell wall of Bacillus subtilis

It is suggested that carboxyl groups provide the major site of metal deposition in the B. subtilis wall, whereas extraction of teichoic acid caused a stoichiometric reduction in levels.

pH dependent charging behavior of isolated cell walls of a gram-positive soil bacterium.

The cell walls of Gram-positive bacteria are highly porous structures that contain many acidic groups, leading to a pH- and salt-dependent charge, and a binding model, the Langmuir-Freundlich equation, is selected which gives a very close reproduction of the observed charging behavior.

Experimental study of uranyl adsorption onto Bacillus subtilis.

Uranyl adsorption onto the Gram-positive soil bacterium Bacillus subtilis was measured using batch experiments in 0.1 M NaClO4 as a function of pH, time, and solid:solute ratio at 25 °C. The

XAFS spectroscopic study of uranyl coordination in solids and aqueous solution

Abstract To evaluate the ability of X-ray absorption fine structure (XAFS) spectroscopy to elucidate the coordination environment of U6+ at the solid-water interface, we conducted an in-depth

Uptake and retention of metals by cell walls of Bacillus subtilis

Isolated walls of Bacillus subtilis Marburg, prepared in a manner which avoided metal contamination other than by the growth medium, were incubated in dilute metal solutions, separated by membrane