X-chromosome inactivation in differentiating mouse embryonic stem cells carrying X-linked GFP and lacZ transgenes.

@article{Farivar2004XchromosomeII,
  title={X-chromosome inactivation in differentiating mouse embryonic stem cells carrying X-linked GFP and lacZ transgenes.},
  author={Shirin Farivar and Shinpei Yamaguchi and Michihiko Sugimoto and Nobuo Takagi},
  journal={The International journal of developmental biology},
  year={2004},
  volume={48 7},
  pages={
          629-35
        }
}
Three new female ES cell lines (GLM1, GLP1 and GLP2) were established from mouse embryos carrying GFP (green fluorescent protein) and HMG-lacZ transgenes on one of two X chromosomes in cis. Using these cell lines, we studied the temporal relationships among three events relevant to X-chromosome inactivation: replication asynchrony of the X chromosome, and quenching of GFP fluorescence and beta-galactosidase (beta-gal) activity, during cell differentiation induced by embryoid body (EB) formation… CONTINUE READING

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