X-aptamers: a bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding.

@article{He2012XaptamersAB,
  title={X-aptamers: a bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding.},
  author={Weiguo He and Miguel-Angel Elizondo-Riojas and Xin Li and Ganesh Lakshmana Rao Lokesh and Anoma D Somasunderam and Varatharasa Thiviyanathan and David E Volk and Ross H Durland and Johnnie Englehardt and Claudio N. Cavasotto and David G. Gorenstein},
  journal={Biochemistry},
  year={2012},
  volume={51 42},
  pages={
          8321-3
        }
}
By combining pseudorandom bead-based aptamer libraries with conjugation chemistry, we have created next-generation aptamers, X-aptamers (XAs). Several X-ligands can be added in a directed or random fashion to the aptamers to further enhance their binding affinities for the target proteins. Here we describe the addition of a drug (N-acetyl-2,3-dehydro-2-deoxyneuraminic acid), demonstrated to bind to CD44-HABD, to a complete monothioate backbone-substituted aptamer to increase its binding… CONTINUE READING

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Expert Opin

  • X. Yang, N. Li, D. G. Gorenstein
  • Drug Discovery
  • 2011

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