Wuschel overexpression promotes somatic embryogenesis and induces organogenesis in cotton (Gossypium hirsutum L.) tissues cultured in vitro

@article{BouchabkCoussa2013WuschelOP,
  title={Wuschel overexpression promotes somatic embryogenesis and induces organogenesis in cotton (Gossypium hirsutum L.) tissues cultured in vitro},
  author={Oumaya Bouchabk{\'e}-Coussa and M. Obellianne and Daphn{\'e} Linderme and {\'E}milie Montes and Alessandra Maia-Grondard and Françoise Vilaine and C. Pannetier},
  journal={Plant Cell Reports},
  year={2013},
  volume={32},
  pages={675-686}
}
Key messageThis work shows that overexpression of theWUSgene fromArabidopsisenhanced the expression of embryogenic competence and triggered organogenesis from some cells of the regenerated embryo-like structures.AbstractAgrobacterium-mediated genetic transformation of cotton was described in the late 1980s, but is still time consuming and largely genotype dependant due to poor regeneration. To help solve this bottleneck, we over-expressed the WUSCHEL (WUS) gene, a homeobox transcription factor… 
AtWuschel Promotes Formation of the Embryogenic Callus in Gossypium hirsutum
TLDR
Ectopic expression of AtWus in CRI12 resulted in upregulation of GhPIN7, GhSHY2, GhLEC1,GhLEC2, and GhFUS3, which may increase the differentiation potential of cotton callus by triggering the auxin transport and signaling pathways.
WUSCHEL Overexpression Promotes Callogenesis and Somatic Embryogenesis in Medicago truncatula Gaertn
TLDR
The effect of the heterologous Arabidopsis WUS gene overexpression under the control of the jasmonate responsive vsp1 promoter on the morphogenic responses of Medicago truncatula explants is investigated.
Ectopic Expression of WUSCHEL (AtWUS) Gene Alters Plant Growth and Development in Rice
TLDR
The seeds from the hemizygous plants showed enhanced embryogenic calli formation and attained early regeneration compared to U and V plants thereby confirming the role of the WUS gene in embryogenesis and regeneration.
Constitutive Overexpression of a Conifer WOX2 Homolog Affects Somatic Embryo Development in Pinus pinaster and Promotes Somatic Embryogenesis and Organogenesis in Arabidopsis Seedlings
Although full sequence data of several embryogenesis-related genes are available in conifers, their functions are still poorly understood. In this study, we focused on the transcription factor
Early expression of WUSCHEL is a marker for in vitro shoot morphogenesis in tobacco and Beta palonga
TLDR
The results indicated that early WUS gene expression is a definite marker for in vitro shoot organogenesis in tobacco and Beta both in direct and indirect modes of regeneration.
Enhanced somatic embryogenesis in Theobroma cacao using the homologous BABY BOOM transcription factor
TLDR
The use of the transcription factor Baby Boom (BBM) to promote the transition of somatic cacao cells from the vegetative to embryonic state is described to provide validation that TcBBM is an ortholog to AtBBM and has a specific role in both somatic and zygotic embryogenesis.
Improving the Efficiency of Adventitious Shoot Induction and Somatic Embryogenesis via Modification of WUSCHEL and LEAFY COTYLEDON 1
TLDR
It is shown that modifying the transcriptional repression/activation activities of WUS and LEC1 improves the efficiency of adventitious organ formation in Arabidopsis.
Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression
TLDR
It was shown that MtWOX9-1 overexpression led to the activation of several groups of genes, including genes related to cell division, tissue diff erentiation, and seed development, and enriched GO pathways included several groups related to histone methyltransferase activity as well as DNA methylation and chromatin binding, suggesting major epigenetic changes that occur in call overexpressing MtW Oxford 9-1.
The WUSCHEL-related homeobox transcription factor MtWOX9-1 stimulates somatic embryogenesis in Medicago truncatula
TLDR
The data obtained suggest that stimulation of MtWOX9-1 expression may have positive impact on plant biotechnology in improving the transformation and regeneration capacity of recalcitrant plants.
Expression of homeobox genes during in vitro culture of Lactuca sativa
TLDR
Findings suggest a key role of LsWUS2L and LsSTM1L genes in the rapid recruitment of the founder cells of adventitious structures in lettuce is suggested.
...
...

References

SHOWING 1-10 OF 82 REFERENCES
Hormonally regulated overexpression of Arabidopsis WUS and conifer LEC1 (CHAP3A) in transgenic white spruce: implications for somatic embryo development and somatic seedling growth
TLDR
Ectopic gene expression was induced by 17-β-estradiol during stages of somatic embryogenesis (early embryogenesis and late embryogenesis) and somatic seedling growth in the transgenics and a high expression of the trans genes did not influence expression profiles of any of the ten other transcription factors tested, some of which have been known to be involved in the process of embryogenesis.
Characterization of leafy cotyledon1-like during embryogenesis in Theobroma cacao L.
TLDR
A homologue of the leafy cotyledon1-like gene, that encodes the HAP 3 subunit of the CCAAT box-binding factor, was found in the cocoa genome (TcL1L).
Overexpression of WUSCHEL in C. chinense causes ectopic morphogenesis
TLDR
An in vitro transformation of Capsicum chinense via Agrobacterium tumefaciens co-cultivation with a system that expresses the heterologous gene WUSCHEL from Arabidopsis thaliana suggests that heterologus W USCHEL was active and involved in the process of morphogenesis.
Expression and Maintenance of Embryogenic Potential Is Enhanced through Constitutive Expression of AGAMOUS-Like 151
TLDR
Results indicate that AGL15 is involved in support of development in an embryonic mode and localization to the nuclei supports a role in gene regulation during this phase of the life cycle.
Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.)
TLDR
A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation and a high percentage of infertile plants was observed among the regenerants.
Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro.
TLDR
It appeared that the xylem pericycle is more pluripotent than previously thought, and was accompanied by the ability of pericycle derivatives to retain diploidy, even after several rounds of cell division, while the phloem pericycle did not display such developmental plasticity.
Ectopic Expression of BABY BOOM Triggers a Conversion from Vegetative to Embryonic Growth Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001941.
TLDR
The expression pattern of BABY BOOM in developing seeds combined with the BBM overexpression phenotype suggests a role for this gene in promoting cell proliferation and morphogenesis during embryogenesis.
Expression of WUSCHEL in Coffea canephora causes ectopic morphogenesis and increases somatic embryogenesis
TLDR
The results show that transgenic expression of the transcription factor WUS can be useful to increase somatic embryogenesis in heterologous systems, however, a critical developmental stage and additional hormonal requirements are required for the induction of embryogenesis by WUS in Coffea canephora.
Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants
TLDR
Cotton (Gossypium hirsutum L.) cotyledon tissues have been efficiently transformed and plants have been regenerated, facilitating transfer of genes of economic importance to cotton.
Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.)
TLDR
It seems that a high 2iP:auxin ratio is preferred for callus initiation and proliferation, but should be exchanged with a higher NAA:cytokinin ratio before differentiation will occur.
...
...