Wound re-epithelialization: modulating keratinocyte migration in wound healing.

  title={Wound re-epithelialization: modulating keratinocyte migration in wound healing.},
  author={Raja and K Sivamani and Miki Shirakawa Garcia and Roslyn Rivkah Isseroff},
  journal={Frontiers in bioscience : a journal and virtual library},
An essential feature of a healed wound is the restoration of an intact epidermal barrier through wound epithelialization, also known as re-epithelialization. The directed migration of keratinocytes is critical to wound epithelialization and defects in this function are associated with the clinical phenotype of chronic non-healing wounds. A complex balance of signaling factors and surface proteins are expressed and regulated in a temporospatial manner that promote keratinocyte motility and… 

Epithelialization in Wound Healing: A Comprehensive Review.

The pivotal role of keratinocytes in epithelialization is focused on, including cellular processes and mechanisms of their regulation during re-epithelialization, and their cross talk with other cell types participating in wound healing.

Re-epithelialization of adult skin wounds: Cellular mechanisms and therapeutic strategies.

Lessons From Epithelialization: The Reason Behind Moist Wound Environment

Moist wound environment enhances the epithelialization process by easier migration of epidermal cells, faster epithelization, and prolonged presence of proteinases and growth factors, which is known to be influenced by the wound environment where moist wound environment is preferred rather than dry wound environment.

The Roles of Growth Factors in Keratinocyte Migration.

This review will highlight the growth factors, particularly transforming growth factor-α (TGF-α), heparin-binding epidermal growth factor (HB-EGF), insulin-like growth factor 1 (IGF-1), fibroblast growth factor 7 (FGF-7), FGF-10, and hepatocyte growthFactor (HGF), which have conclusively been shown to be the most motogenic for KCs.

Roles of lactoferrin on skin wound healing.

  • Y. TakayamaR. Aoki
  • Biology, Medicine
    Biochemistry and cell biology = Biochimie et biologie cellulaire
  • 2012
In an in vitro model of wound contraction, lactoferrin promoted fibroblast-mediated collagen gel contraction and indicates that lact oferrin supports multiple biological processes involved in wound healing.

Extracellular matrix contribution to skin wound re-epithelialization.

Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing

The rescue of Dock5 expression in diabetic mice causes a significant improvement in reepithelialization, collagen deposition, ECM production, and granulation, providing a potential therapeutic target for wound healing impairment during diabetes.

Src promotes cutaneous wound healing by regulating MMP-2 through the ERK pathway

Evidence is provided that Src promotes keratinocyte migration and cutaneous wound healing, in which the regulation of MMP-2 through the ERK pathway plays an important role, and a potential therapeutic role is demonstrated for Src in cutaneous wounds healing.

The involvement of extracellular matrix proteins in the re-epithelialization of skin wounds

The ability of skin to act as a barrier is primarily determined by cells that maintain the continuity and integrity of skin and restore it after injury. Cutaneous wound healing in adult mammals is a

Establishment of a novel in vitro model of stratified epithelial wound healing with barrier function

A straightforward in vitro wound assay to evaluate the healing and restoration of barrier function in stratified human corneal epithelial cells and the closure of the wound was associated with the restoration of the transcellular barrier and the re-establishment of apical intercellular junctions.



Contributions of the epidermal growth factor receptor to keratinocyte motility

It is proposed that transient and dynamic elevation of EGF receptor during wound healing, or constitutive overexpression in tumors, provides an important contribution to the migratory and invasive potential of keratinocytes.

Numerous keratinocyte subtypes involved in wound re-epithelialization.

Immunohistochemistry with specific anti-keratin monoclonal and polyclonal antibodies was used to examine AW in normal healthy volunteers and found that keratinocytes populated the wound bed below the scab by migration, supported by keratinocyte proliferation in the surrounding epidermis both at and adjacent to the wound edge.

Migration of epidermal keratinocytes: mechanisms, regulation, and biological significance

The data reviewed here point to a sophisticated cooperation between soluble motogenic growth factors, cell–matrix interactions, and cell-to-cell communications as major parts of the machinery regulating keratinocyte migration.

Epidermal expression of collagenase delays wound-healing in transgenic mice.

Results are consistent with the hypothesis that control of collagenase (MMP-1) expression is important for re-epithelialization during wound healing and indicate that collagenase regulation is critical to the kinetics of normal wound closure.

A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair.

A crucial role of beta 1 integrins in keratinocyte migration and wound re-epithelialisation is demonstrated and their proliferation rate was not reduced in early wounds and even increased in late wounds.

TGF-beta 1 stimulates expression of keratinocyte integrins during re-epithelialization of cutaneous wounds.

During wound repair, TGF-beta 1 may induce epidermal keratinocytes to express integrins that facilitate the migratory component of re-epithelialization.

Hypoxia increases human keratinocyte motility on connective tissue.

It is demonstrated that hypoxia promotes human keratinocyte motility on connective tissue and is associated with increased expression of lamellipodia proteins, increase expression of collagenase and decreased expression of laminin-5, the locomotion brake for keratinocytes.

Upregulation of α9 Integrin and Tenascin During Epithelial Regeneration After Debridement in the Cornea

  • M. SteppL. Zhu
  • Biology
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 1997
Using a debridement wound model in the mouse cornea, α9 integrin protein production and tenascin accumulation are dynamically regulated in response to corneal epithelial injury.

Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelialization.

It is demonstrated that epidermal cells do move over a fibrin and fibronectin matrix during in vivo wound repair, as well as other factors regulating the attachment and directional migration of a regenerating epidermis in wound healing.

Bioactive interleukin-8 is expressed in wounds and enhances wound healing.

Results indicate the sequential function of endogenous IL-8 in all phases of human wound healing and suggest it may be useful in impaired wound healing.