Wound hypoxia and acidosis limit neutrophil bacterial killing mechanisms.

  title={Wound hypoxia and acidosis limit neutrophil bacterial killing mechanisms.},
  author={David B. Allen and Janet J. Maguire and Mani Mahdavian and Corinna Wicke and L Marcocci and Heinz Scheuenstuhl and Moon-Seok Chang and A X Le and Harriet W. Hopf and Thomas K. Hunt},
  journal={Archives of surgery},
  volume={132 9},
BACKGROUND "Respiratory burst" activity, ie, O2- production, is dependent on PO2, temperature, pH, and glucose concentrations within the physiologic range. OBJECTIVES To determine whether environmental conditions characteristic of wounds may limit human neutrophil respiratory burst metabolism and to clarify the degree to which bactericidal oxidant production depends on local PO2. METHODS Human blood and wound neutrophils were stimulated with phorbol myristate acetate. Oxygen consumption and… CONTINUE READING