Wortmannin inhibits proliferation and induces apoptosis of MCF-7 breast cancer cells.

@article{Yun2012WortmanninIP,
  title={Wortmannin inhibits proliferation and induces apoptosis of MCF-7 breast cancer cells.},
  author={Jun No Yun and Ying Guo Lv and Qing Yao and Lingxiao Wang and Yi Li and Juanjuan Yi},
  journal={European journal of gynaecological oncology},
  year={2012},
  volume={33 4},
  pages={367-9}
}
OBJECTIVE The present study aimed to explore the effects of wortmannin in the proliferation and apoptosis of human breast cancer MCF-7 cells. METHODS The authors treated cells with 0, 1, 6.25, 12.5, 25, and 50 nM wortmannin for 24, 48, and 72 hours. Inhibition of proliferation was measured by cell counting kit-8 assay (CCK8). Apoptosis was detected with Annexin V-fluorescein isothiocyanate/propidium iodide double staining by flow cytometry. Additionally, expression of proteins involved in the… CONTINUE READING