Wnt/Wingless Signaling Requires BCL9/Legless-Mediated Recruitment of Pygopus to the Nuclear β-Catenin-TCF Complex

@article{Kramps2002WntWinglessSR,
  title={Wnt/Wingless Signaling Requires BCL9/Legless-Mediated Recruitment of Pygopus to the Nuclear $\beta$-Catenin-TCF Complex},
  author={Thomas Kramps and Oliver Peter and Erich Brunner and Denise Nellen and Barbara A. Froesch and Sandipan Chatterjee and Maximilien Murone and Stephanie Z{\"u}llig and Konrad Basler},
  journal={Cell},
  year={2002},
  volume={109},
  pages={47-60}
}
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pygopus Encodes a nuclear protein essential for wingless/Wnt signaling.
TLDR
It is shown that Wg signaling is diminished during embryogenesis and imaginal disc development in the absence of pygo activity, and Pygo is likely to act as a transcription co-activator required for the nuclear function of Arm/beta-catenin.
BCL9-2 binds Arm/β-catenin in a Tyr142-independent manner and requires Pygopus for its function in Wg/Wnt signaling
Dissecting nuclear Wingless signalling: Recruitment of the transcriptional co-activator Pygopus by a chain of adaptor proteins
Evidence that Armadillo Transduces Wingless by Mediating Nuclear Export or Cytosolic Activation of Pangolin
Wingless-Independent Association of Pygopus with dTCF Target Genes
Pygopus and Legless Provide Essential Transcriptional Coactivator Functions to Armadillo/β-Catenin
Pygopus and Legless target Armadillo/β-catenin to the nucleus to enable its transcriptional co-activator function
TLDR
It is shown that the nuclear localization of Lgs entirely depends on Pygo, which itself is constitutively localized to the nucleus; thus, Pygo functions as a nuclear anchor.
Constitutive scaffolding of multiple Wnt enhanceosome components by Legless/BCL9
TLDR
An unexpected link between BCL9/B9L, PYGO2 and nuclear co-receptor complexes suggests that these β-catenin co-factors may coordinate Wnt and nuclear hormone responses.
Identification and in vivo role of the Armadillo-Legless interaction
TLDR
It is inferred that the β-catenin/Lgs binding site may serve as an attractive drug target for therapeutic intervention in β- catenin-dependent cancer progression.
Pygopus Residues Required for its Binding to Legless Are Critical for Transcription and Development*
TLDR
Four conserved residues at the putative PHD domain surface of Drosophila and mouse Pygopus that are required for their binding to Legless in vitro and in vivo are identified and defined as a specific molecular target for blocking Wnt signaling during development and cancer.
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