Wirkung von Dauno-Rubidomycin und Adriamycin auf Enzyme der DNS-Synthese in Leukocyten in vivo und in der Kultur

  title={Wirkung von Dauno-Rubidomycin und Adriamycin auf Enzyme der DNS-Synthese in Leukocyten in vivo und in der Kultur},
  author={K. Wilms and Wolfgang Wilmanns},
  journal={Klinische Wochenschrift},
SummaryThe enzyme activities of DNA polymerase and thymidine kinase were determined in peripheral leukemic leukocytes of patients with acute leukemia treated with Dauno-Rubidomycin or Adriamycin. During the first hours after the injection of each drug both enzymes are significantly augmented. After a few days there is a distinct decrease of the enzyme activities preceding the fall of the leukocyte count.The initial increase in enzyme activity could be reproduced in PHA stimulated lymphocyte… 

Comparative studies of the incorporation inhibition of radioactive nucleotides and amino acids in vitro under the influence of adriamycin, bleomycin, and sodium arsenate

It was shown by means of cytophoto metrical examinations that Adriamycin is capable of fixing cells in the G2 phase and in the S phase, and that the inhibition caused by Bleomycin and arsenate is considerably less destructive.

Methylcholanthrene induced murine primitive neuroectodermal tumor: ultrastructure and nuclear RNA polymerase activity

The histology, ultrastructure, and nuclear RNA polymerase activity are described in a murine primitive neuroectodermal tumor derived by serial transplantation from a tumor originally induced with methylcholanthrene and classified as an ependymoblastoma, which suggests that the RNA polymerases inhibited by alpha amanitin contribute less to the total nuclear RNA synthesis.

Experimental investigations on a sequential combination chemotherapy protocol

It was found that the sequential combination of HU and VCR caused the greatest alteration of growth pattern compared with other combination protocols, and the administration of ADM at the time of DNA synthesis of the partially synchronized cells resulted in a statistically significant prolongation of the mean survival time and in 30% of cures of the animals.



Action of Daunomycin on Nucleic Acid Synthesis in Hepatoma Ascites Cells Incubated « in vitro »

The action of Dannomycin, an antibiotic of Rhodomycin group, was studied following the incorporation of Adenine-8-14C into nucleic acid of hepatoma ascites cells incubated « in vitro »; the activity of the drug appeared to be dependent on the density of the cell population.

Daunomycin inhibition of DNA and RNA synthesis

Observations indicate that Daunomycin is a more effective inhibitor of the synthesis of DNA than RNA, and hence the biologic mechanism of action of this antibiotic is probably different from that of actinomycin.

Daunomycin, an antitumor antibiotic, in the treatment of neoplastic disease. Clinical evaluation with special reference to childhood leukemia

Daunomycin is temporarily effective in some cases of neuroblastoma, reticulum cell sarcoma and rhabdomyosarcoma, and in patients receiving maintenance doses of dauncycin the development of tachypnea, tachycardia pulmonary insufficiency, heart failure and hypotension possibly is associated with daunomcin but the evidence is unclear.

The effect of daunomycin on human cells in vivo and in vitro

In‐vitro studies on normal human lymphocytes indicate that daunomycin affects RNA and DNA synthesis and that it interferes with the cell cycle during the G2 period and appears to have the unique capacity to delay the onset of mitosis in cells which have already synthesized DNA.

Influence of antiblastic agents applied either in vivo or in vitro on human lymphocyte transformation in cell culture

The present experiments show that antimitotic agents commonly used in the treatment of neoplastic disorders do not behave uniformly with respect to the action on the blastic transformation of lymphocytes, and should be kept in mind, whenever these agents are given for a clinical treatment.

Daunorubicine in acute myelogenous leukemia in adults.

This drug appears to be an effective therapy for the induction of remissions in adults with acute myelogenous leukemia, and maintenance therapy with other chemotherapeutic agents has been employed.


Die Thymidin-Kinase in normalen und leukämischen myeloischen Zellen

Enzymatic investigations of blood cells show the following relations between both pathways: in mature leucocytes of the peripheral blood the FH2-reductase is not detectable and the activity of thymidine kinase is very low.

Phase I and preliminary phase II evaluation of adriamycin (NSC 123127).

Adriamycin produced consistent regressions in ten histologically different types of neoplastic disease, but mostly in acute lymphoblastic and chronic (myeloid and lymphocytic) leukemias, as well as in all types of malignant lymphomas, neuroblastoma, Ewing sarcoma, soft-tissue sarcomas, and chorioepithelioma of the uterus.

Base Specificity in the Interaction of Polynucleotides with Antibiotic Drugs

Chromomycin and olivomycin form complexes with DNA, preferably in the helical form, but not with RNA, and none of the other antibiotics requires the presence of any single base in the template for its action.