Wild-type but not mutant p53 immunopurified proteins bind to sequences adjacent to the SV40 origin of replication.

@article{Bargonetti1991WildtypeBN,
  title={Wild-type but not mutant p53 immunopurified proteins bind to sequences adjacent to the SV40 origin of replication.},
  author={Jill Bargonetti and Paula N. Friedman and Scott E. Kern and Bert Vogelstein and Carol Prives},
  journal={Cell},
  year={1991},
  volume={65 6},
  pages={1083-91}
}
The DNA from a wide variety of human tumors has sustained mutations within the conserved p53 coding regions. We have purified wild-type and tumor-derived mutant p53 proteins expressed from baculovirus vectors and examined their interactions with SV40 DNA. Using DNAase I footprinting assays, we observed that both human and murine wild-type p53 proteins bind specifically to sequences adjacent to the late border of the viral replication origin. By contrast, mutant p53 proteins failed to bind… CONTINUE READING

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