Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens

@article{Dominguez2005WildtypeBP,
  title={Wild-type blocking polymerase chain reaction for detection of single nucleotide minority mutations from clinical specimens},
  author={Patrick Lee Dominguez and Michael S. Kolodney},
  journal={Oncogene},
  year={2005},
  volume={24},
  pages={6830-6834}
}
Detection and sequencing of mutations from clinical specimens is often complicated by the presence of an excess of nonmutated cells. To facilitate the detection and sequencing of minority mutations from clinical specimens, we developed wild-type blocking polymerase chain reaction (WTB-PCR). This technique allows sensitive detection of minority mutations in a tissue sample containing excess wild-type DNA. In WTB-PCR, a nonextendable locked nucleic acid (LNA) oligonucleotide binds tightly to a… CONTINUE READING
Highly Cited
This paper has 64 citations. REVIEW CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 35 extracted citations

64 Citations

051015'08'11'14'17
Citations per Year
Semantic Scholar estimates that this publication has 64 citations based on the available data.

See our FAQ for additional information.

References

Publications referenced by this paper.
Showing 1-6 of 6 references

J

CJ Miller, M Cheung, +4 authors GP. Robertson
Invest. Dermatol., 123, 990–992. • 2004
View 4 Excerpts
Highly Influenced

Hum

Makrigiorgos GM.
Mutat., 23, 406–412. • 2004

Expert Rev

P Mouritzen, AT Nielsen, +3 authors S. Moller
Mol. Diagn., 3, 27–38. • 2003

Mol

D Latorra, K Arar, JM. Hurley
Cell Probes, 17, 253–259. • 2003
View 2 Excerpts

J

DH Oliver, RE Thompson, CA Griffin, JR. Eshleman
Mol. Diagn., 2, 202–208. • 2000

Mutat

BL Parsons, Heflich RH.
Res., 387, • 1997