Why does ribonuclease irreversibly inactivate at high temperatures?

@article{Zale1986WhyDR,
  title={Why does ribonuclease irreversibly inactivate at high temperatures?},
  author={Stephen E. Zale and Alexander M. Klibanov},
  journal={Biochemistry},
  year={1986},
  volume={25 19},
  pages={5432-44}
}
The mechanism of irreversible thermoinactivation of bovine pancreatic ribonuclease A in the pH range relevant to enzymatic catalysis has been elucidated. At 90 degrees C and pH 4, the enzyme inactivation is caused by hydrolysis of peptide bonds at aspartic acid residues (the main process) and deamidation of asparagine and/or glutamine residues. At 90 degrees C and neutral pH (pH 6 and 8), the enzyme inactivation is caused by a combination of disulfide interchange (the main process), beta… CONTINUE READING

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