Whole transcriptome characterization of aberrant splicing events induced by lentiviral vector integrations.

@article{Cesana2012WholeTC,
  title={Whole transcriptome characterization of aberrant splicing events induced by lentiviral vector integrations.},
  author={Daniela Cesana and Jacopo Sgualdino and Laura Rudilosso and Stefania Merella and Luigi Naldini and Eugenio Montini},
  journal={The Journal of clinical investigation},
  year={2012},
  volume={122 5},
  pages={1667-76}
}
Gamma-retroviral/lentiviral vectors (γRV/LV) with self-inactivating (SIN) long terminal repeats (LTRs) and internal moderate cellular promoters pose a reduced risk of insertional mutagenesis when compared with vectors with active LTRs. Yet, in a recent LV-based clinical trial for β-thalassemia, vector integration within the HMGA2 gene induced the formation of an aberrantly spliced mRNA form that appeared to cause clonal dominance. Using a method that we developed, cDNA linear amplification… CONTINUE READING

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