Whole-muscle and single-fibre contractile properties and myosin heavy chain isoforms in humans

@article{Harridge2009WholemuscleAS,
  title={Whole-muscle and single-fibre contractile properties and myosin heavy chain isoforms in humans},
  author={Stephen D. R. Harridge and Roberto Bottinelli and Monica Canepari and Maria Antonietta Pellegrino and Carlo Reggiani and Mona Esbj{\"o}rnsson and Bengt Saltin},
  journal={Pfl{\"u}gers Archiv},
  year={2009},
  volume={432},
  pages={913-920}
}
The contractile characteristics of three human muscle groups (triceps surae, quadriceps femoris and triceps brachii) of seven young male subjects were examined. The contractile properties were determined from electrically evoked isometric responses and compared with fibre type composition determined from needle biopsy samples. Fibre types were identified using myosin heavy chain (MHC) isoforms as molecular markers with gel electrophoresis (SDS-PAGE) and histochemical ATPase staining. Four… Expand
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References

SHOWING 1-10 OF 45 REFERENCES
Maximum velocity of shortening in relation to myosin isoform composition in single fibres from human skeletal muscles.
TLDR
Maximum tension (P0) and specific tension were lower in freeze‐dried types I and IIA fibres than in chemically skinned, while no differences were observed in the IIA/B fibres. Expand
Force‐velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle.
TLDR
The results suggest that MHC composition is just one of the determinants of shortening velocity and of other muscle contractile properties. Expand
Unloaded shortening velocity and myosin heavy chain and alkali light chain isoform composition in rat skeletal muscle fibres.
TLDR
The maximum velocity of shortening as determined by the slack test (Vo) was tested for its relationship with MHC composition and with alkali MLC isoform ratio of fast fibres of known M HC composition and was found to be proportional to the relative content of MLC3f in IIA, IIX and IIB fibres. Expand
Calcium sensitivity and myofibrillar protein isoforms of rat skinned skeletal muscle fibres
TLDR
It is suggested that when both fast and slow isoforms of regulatory proteins and of MLC are present in a muscle fibre, calcium sensitivity is dictated mainly by the fast isoforms. Expand
Correlation between percentage fiber type area and myosin heavy chain content in human skeletal muscle
TLDR
Correlation analyses suggest a relationship between the histochemically assessed percentage fiber type area and the electrophoretically assessed MHC content in human limb musculature. Expand
Ageing alters the myosin heavy chain composition of single fibres from human skeletal muscle.
TLDR
Results indicate that coexistence of myosin heavy chain isoforms in single fibres is present in skeletal muscles of young adults, and that there is an increased occurrence of this phenomenon with ageing. Expand
Analysis of myosin light and heavy chain types in single human skeletal muscle fibers.
TLDR
In normal human muscle a number of 'hybrid' myosins were found to occur, namely two extreme forms of fast myosin which have the same light chains but different heavy chains (IIA and IIB) and a continuum of slow forms consisting of the same heavy chain and slow light chains with a variable fast light chain composition. Expand
Characterization of human skeletal muscle fibres according to the myosin heavy chains they express
TLDR
It was shown that the human equivalent to the rat type IIX MyoHC gene is expressed and this observation was taken to suggest that the previously classified IIB muscle fibres in human muscle express a Myosin heavy chain isoform equivalent toThe rat IIX, not the IIB, and would therefore be more accurately classified as IIX fibres. Expand
Myosin heavy chain isoforms in single fibres from m. vastus lateralis of soccer players: effects of strength-training.
TLDR
It is suggested that nearly all histochemical type IIB fibres of the soccer players display co-existence of both MHC isoform IIA and IIB, as well as one-dimensional electrophoresis on muscle homogenates, which suggested no major change in the muscle fibre area of the two groups. Expand
Myosin heavy chain isoforms in single fibres from m. vastus lateralis of sprinters: influence of training.
TLDR
The majority of histochemically determined type IIB fibres of sprinters seems to contain both MHC isoforms IIA and IIB, and Sprint-training appears to induce an increased expression of MHCisoform IIA in skeletal muscles. Expand
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