Whole mount immunohistochemistry and in situ hybridization of larval and adult zebrafish dental tissues.

@article{Verstraeten2012WholeMI,
  title={Whole mount immunohistochemistry and in situ hybridization of larval and adult zebrafish dental tissues.},
  author={Barbara Verstraeten and Ellen Sanders and Ann Huysseune},
  journal={Methods in molecular biology},
  year={2012},
  volume={887},
  pages={179-91}
}
Tooth development is increasingly being studied in a variety of vertebrate model organisms, each contributing its own perspective to our understanding of dental diversity. In situ hybridization and immunohistochemistry are well-established and frequently used techniques to study the presence of mRNA and protein. Here, we describe a protocol for whole mount immunohistochemistry and in situ hybridization that can be applied to all stages of zebrafish development and dissected bony parts. The… CONTINUE READING
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