Whole genomic sequence analysis of Bacillus infantis: defining the genetic blueprint of strain NRRL B-14911, an emerging cardiopathogenic microbe

  title={Whole genomic sequence analysis of Bacillus infantis: defining the genetic blueprint of strain NRRL B-14911, an emerging cardiopathogenic microbe},
  author={Chandirasegaran Massilamany and Akram Mohammed and John Dustin Loy and Tanya J Purvis and Bharathi Krishnan and Rakesh H. Basavalingappa and Christy M. Kelley and Chittibabu Guda and Raúl G. Barletta and Etsuko N. Moriyama and Timothy P. L. Smith and Jay Reddy},
  journal={BMC Genomics},
BackgroundWe recently reported the identification of Bacillus sp. NRRL B-14911 that induces heart autoimmunity by generating cardiac-reactive T cells through molecular mimicry. This marine bacterium was originally isolated from the Gulf of Mexico, but no associations with human diseases were reported. Therefore, to characterize its biological and medical significance, we sought to determine and analyze the complete genome sequence of Bacillus sp. NRRL B-14911.ResultsBased on the phylogenetic… 
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Bacillus infantis sp. nov. and Bacillus idriensis sp. nov., isolated from a patient with neonatal sepsis.
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    International journal of systematic and evolutionary microbiology
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Two Gram-positive bacilli, isolated sequentially from the blood of a newborn child with sepsis, were found to be closely related to Bacillus firmus NCIMB 9366T and Bacillus cibi JG-30T and represent two novel species of the genus Bacillus.
Reassessment of Sequence-Based Targets for Identification of Bacillus Species
The high 16S rRNA gene sequence similarities between some strains demonstrated the need for a polyphasic approach to the systematics of this genus, and the 16S-23S spacer region proved discriminatory between most species.
Discovery of marine Bacillus species by 16S rRNA and rpoB comparisons and their usefulness for species identification.
It is demonstrated that the polymorphism of the Bacillus rpoB gene can be used to identify Bacillus species, providing an improved identification scheme for Bacilli species.
Bacillus anthracis Diverges from Related Clades of the Bacillus cereus Group in 16S-23S Ribosomal DNA Intergenic Transcribed Spacers Containing tRNA Genes
Mung bean nuclease treatment of 16S-23S ribosomal DNA intergenic transcribed spacers (ITS) amplified from several strains of the six species of the Bacillus cereus group showed that B. cereus and B. thuringiensis represent a single clade, indicating that the anthrax agent started evolving separately from the related clades of the B. Cereus group.
PCR fingerprinting of whole genomes: the spacers between the 16S and 23S rRNA genes and of intergenic tRNA gene regions reveal a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniformis [corrected].
From data, it is hypothesized that two different evolutionary schemes for the two species can arise from plasmid transfer, and a wide variability among the B. cereus strains was also observed in thePlasmid profiles, suggesting that the genetic diversity within B. Cereus species can arises from plasmsid transfer.
Phylogeny of Marine Bacillus Isolates from the Gulf of Mexico
Only NRRL B-14851, an unusually thin rod that forms very small spores, may represent a new Bacillus species.
Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus.
A comparison of 16S rRNA gene sequences was made among type strains of 69 Bacillus species approved in the International Journal of Systematic Bacteriology until 1998 and revealed that this region was highly conserved within the species.
VFDB 2012 update: toward the genetic diversity and molecular evolution of bacterial virulence factors
This article presents the recent major updates of VFDB in an attempt to summarize some of the most important virulence mechanisms by comparing different compositions and organizations of VFs from various bacterial pathogens, identifying core components and phylogenetic clades and shedding new light on the forces that shape the evolutionary history of bacterial pathogenesis.
Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences.
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  • Biology, Medicine
    International journal of systematic and evolutionary microbiology
  • 2003
The phylogenetic classification presented here is in general in agreement with current classifications based on phenotypic and molecular data, but the findings suggest, however, that in some cases, further divisions or, conversely, further groupings might be warranted.
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