Whole-genome sequencing and microarray analysis of ex vivo Plasmodium vivax reveal selective pressure on putative drug resistance genes

  title={Whole-genome sequencing and microarray analysis of ex vivo Plasmodium vivax reveal selective pressure on putative drug resistance genes},
  author={Neekesh V. Dharia and Andrew Bright and Scott Westenberger and S. Whitney Barnes and Sergei Batalov and Kelli L. Kuhen and Rachel Borboa and Glenn C Federe and Colleen M. McClean and Joseph Michael Vinetz and Victor Neyra and Alejandro Llanos-Cuentas and John W. Barnwell and John R. Walker and Elizabeth A. Winzeler},
  journal={Proceedings of the National Academy of Sciences},
  pages={20045 - 20050}
Plasmodium vivax causes 25–40% of malaria cases worldwide, yet research on this human malaria parasite has been neglected. Nevertheless, the recent publication of the P. vivax reference genome now allows genomics and systems biology approaches to be applied to this pathogen. We show here that whole-genome analysis of the parasite can be achieved directly from ex vivo-isolated parasites, without the need for in vitro propagation. A single isolate of P. vivax obtained from a febrile patient with… 

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