Whole-genome microarrays of fission yeast: characteristics, accuracy, reproducibility, and processing of array data

@article{Lyne2003WholegenomeMO,
  title={Whole-genome microarrays of fission yeast: characteristics, accuracy, reproducibility, and processing of array data},
  author={Rachel Lyne and Gavin Burns and Juan Mata and Chris J Penkett and Gabriella Rustici and Dongrong Chen and Cordelia Langford and David Vetrie and J{\"u}rg B{\"a}hler},
  journal={BMC Genomics},
  year={2003},
  volume={4},
  pages={27 - 27}
}
The genome of the fission yeast Schizosaccharomyces pombe has recently been sequenced, setting the stage for the post-genomic era of this increasingly popular model organism. We have built fission yeast microarrays, optimised protocols to improve array performance, and carried out experiments to assess various characteristics of microarrays. We designed PCR primers to amplify specific probes (180–500 bp) for all known and predicted fission yeast genes, which are printed in duplicate onto… CONTINUE READING