Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay

@article{Tombcz2009WholegenomeAO,
  title={Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay},
  author={D{\'o}ra Tomb{\'a}cz and Judit S. T{\'o}th and P{\'a}l Petrovszki and Zsolt Boldogkői},
  journal={BMC Genomics},
  year={2009},
  volume={10},
  pages={491 - 491}
}
Pseudorabies virus (PRV), a neurotropic herpesvirus of pigs, serves as an excellent model system with which to investigate the herpesvirus life cycle both in cultured cells and in vivo. Real-time RT-PCR is a very sensitive, accurate and reproducible technique that can be used to detect very small amounts of RNA molecules, and it can therefore be applied for analysis of the expression of herpesvirus genes from the very early period of infection. In this study, we have developed and applied a… CONTINUE READING

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