Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm

Abstract

The beauty and power of the genome editing mechanism, CRISPR Cas9 endonuclease system, lies in the fact that it is RNA-programmable such that Cas9 can be guided to any genomic loci complementary to a 20-nt RNA, single guide RNA (sgRNA), to cleave double stranded DNA, allowing the introduction of wanted mutations. Unfortunately, it has been reported… (More)
DOI: 10.1186/s12864-017-4225-1

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@inproceedings{Zhou2017WholeGA, title={Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm}, author={Hong Zhou and Michael Zhou and Daisy Li and Joseph Manthey and Ekaterina Lioutikova and Hong Wang and Xiao Zeng}, booktitle={BMC Genomics}, year={2017} }