Whole-field five-dimensional fluorescence microscopy combining lifetime and spectral resolution with optical sectioning.

@article{Siegel2001WholefieldFF,
  title={Whole-field five-dimensional fluorescence microscopy combining lifetime and spectral resolution with optical sectioning.},
  author={Jan Siegel and Daniel S. Elson and Stephen D. Webb and Duncan Parsons-Karavassilis and Sandrine L{\'e}v{\^e}que-Fort and Martin J. Cole and M. John Lever and Paul M W French and M. A. A. Neil and Rimas Ju{\vs}kaitis and L. O. Sucharov and Tony Wilson},
  journal={Optics letters},
  year={2001},
  volume={26 17},
  pages={1338-40}
}
We report a novel whole-field three-dimensional fluorescence lifetime imaging microscope that incoporates multispectral imaging to provide five-dimensional (5-D) fluorescence microscopy. This instrument, which can acquire a 5-D data set in less than a minute, is based on potentially compact and inexpensive diode-pumped solid-state laser technology. We demonstrate that spectral discrimination as well as optical sectioning minimize artifacts in lifetime determination and illustrate how spectral… CONTINUE READING

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Lakowicz, Principles of Fluorescence Spectroscopy (Plenum

  • J R.
  • New York,
  • 1983
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