Whole cell recordings from respiratory neurones in an arterially perfused in situ neonatal rat preparation.

@article{Dutschmann2003WholeCR,
  title={Whole cell recordings from respiratory neurones in an arterially perfused in situ neonatal rat preparation.},
  author={Mathias Dutschmann and Julian F. R. Paton},
  journal={Experimental physiology},
  year={2003},
  volume={88 6},
  pages={725-32}
}
For synaptic and cellular analyses of the mammalian respiratory network, intracellular recording and good pharmacological access to respiratory neurones is required. Using an existing arterially perfused in situ preparation of neonatal rat, we report on a method allowing stable intracellular recordings of ventrolateral medullary respiratory neurones. The in situ preparation generates a recognizable eupnoeic respiratory motor pattern similar to that reported in vivo. Using this preparation, we… CONTINUE READING

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