Whole-cell patch-clamp recording of recombinant voltage-sensitive Ca2+ channels heterologously expressed in HEK-293 cells.

Abstract

Several factors, including showing a high efficiency of transfection and protein production, faithful translation and processing of proteins, and possession of a small cell size, have together made human embryonic kidney 293 (HEK-293) cells a popular choice among electrophysiologists. This cell line has been extensively used to study recombinant… (More)
DOI: 10.1101/pdb.prot073213

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@article{Gandini2014WholecellPR, title={Whole-cell patch-clamp recording of recombinant voltage-sensitive Ca2+ channels heterologously expressed in HEK-293 cells.}, author={Maria Alejandra Gandini and Alejandro Cruz Sandoval and Ricardo F{\'e}lix}, journal={Cold Spring Harbor protocols}, year={2014}, volume={2014 4}, pages={396-401} }