Whole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin α2 as a Host Factor for Bacterial Invasion

Abstract

To understand the role of glycosaminoglycans in bacterial cellular invasion, xylosyltransferase-deficient mutants of Chinese hamster ovary (CHO) cells were created using clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated gene 9 (CRISPR-cas9) gene targeting. When these mutants were compared to the pgsA745 cell line, a CHO… (More)
DOI: 10.1128/mBio.02128-16

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@inproceedings{Wijk2017WholeGenomeSO, title={Whole-Genome Sequencing of Invasion-Resistant Cells Identifies Laminin α2 as a Host Factor for Bacterial Invasion}, author={Xander M R van Wijk and Simon D{\"o}hrmann and Bj{\"o}rn Mikael Hallstr{\"o}m and Shangzhong Li and Bj\orn Gunnar Voldborg and Brandon X. Meng and K K Mckee and Toin H. van Kuppevelt and Peter D Yurchenco and Bernhard O. Palsson and Nathan E. Lewis and Victor Nizet and Jeffrey D Esko}, booktitle={mBio}, year={2017} }