Whole Genome Amplification and De novo Assembly of Single Bacterial Cells

@inproceedings{Rodrigue2009WholeGA,
  title={Whole Genome Amplification and De novo Assembly of Single Bacterial Cells},
  author={S{\'e}bastien Rodrigue and Rex R Malmstrom and Aaron M. Berlin and Bruce W. Birren and Matthew R. Henn and Sallie W. Chisholm},
  booktitle={PloS one},
  year={2009}
}
BACKGROUND Single-cell genome sequencing has the potential to allow the in-depth exploration of the vast genetic diversity found in uncultured microbes. We used the marine cyanobacterium Prochlorococcus as a model system for addressing important challenges facing high-throughput whole genome amplification (WGA) and complete genome sequencing of individual cells. METHODOLOGY/PRINCIPAL FINDINGS We describe a pipeline that enables single-cell WGA on hundreds of cells at a time while virtually… CONTINUE READING