Whole Cell Cryo-Electron Tomography Suggests Mitochondria Divide by Budding

  title={Whole Cell Cryo-Electron Tomography Suggests Mitochondria Divide by Budding},
  author={G. B. Hu},
  journal={Microscopy and Microanalysis},
  pages={1180 - 1187}
  • G. Hu
  • Published 28 May 2014
  • Biology
  • Microscopy and Microanalysis
Abstract Eukaryotes rely on mitochondrial division to guarantee that each new generation of cells acquires an adequate number of mitochondria. Mitochondrial division has long been thought to occur by binary fission and, more recently, evidence has supported the idea that binary fission is mediated by dynamin-related protein (Drp1) and the endoplasmic reticulum. However, studies to date have depended on fluorescence microscopy and conventional electron microscopy. Here, we utilize whole cell… 

Membrane Bending Energy and Tension Govern Mitochondrial Division

It is proposed that mitochondrial fission is probabilistic, and can be modeled as arising from bending energy complemented by a fluctuating membrane tension, largely arising from pulling forces.

Cellular structural biology as revealed by cryo-electron tomography

Visualization of macromolecules, organelles and cells by cryo-ET allows for structural analysis in a near-native environment, as recent technological advances have revealed unprecedented structural details.

Giant viruses: spore‐like missing links between Rickettsia and mitochondria?

  • H. Seligmann
  • Biology
    Annals of the New York Academy of Sciences
  • 2019
Phyloproteomics indicate common viral origin from ancient cells before archaea, bacteria, and eukaryota split and subsequent size and complexity reductions occurred, implying cellular‐to‐viral‐to-subcellular macroevolution.


  • P. Roy
  • Encyclopedia of Animal Cognition and Behavior
  • 2019



Cryo-electron tomography of neurospora mitochondria.

Cryo-electron tomography was used to study the structural organization of whole frozen-hydrated mitochondria from Neurospora crassa, indicating that it is a realistic goal to achieve "molecular resolution" with rather large biological specimens in the near future, ultimately allowing the identification and localization of macromolecules in their cellular context.

The internal structure of mitochondria.

The machines that divide and fuse mitochondria

  • J. Nunnari
  • Biology
    Annual review of biochemistry
  • 2007
In vitro assays that recapitulate mitochondrial division and fusion events are established and three highly conserved dynamin‐related proteins (DRPs), which are large self‐assembling GTPases that regulate membrane dynamics in a variety of cellular processes are discussed.

Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells.

It is shown that mutations in the human dynamin-related protein Drp1 cause mitochondria to form perinuclear clusters that consist of highly interconnected mitochondrial tubules, which indicates that the balance between mitochondrial division and fusion is shifted toward fusion.

Mitochondrial fusion and fission in cell life and death

The core components of the evolutionarily conserved fusion and fission machineries have now been identified, and mechanistic studies have revealed the first secrets of the complex processes that govern fusion andfission of a double membrane-bound organelle.

Structure of mitochondria and vacuoles of Candida utilis and Schizosaccharomyces pombe studied by electron microscopy of serial thin sections and model building.

The models of the two cells of C. utilis and S. pombe confirmed that there is a single, branched and continuous mitochondrial network in the cell and showed that the mitochondrial structure was far more continuous than expected from inspection of thin sections.

MiD49 and MiD51, new components of the mitochondrial fission machinery

Overexpression of MiD49/51 seems to sequester Drp1 from functioning at mitochondria and cause fused tubules to associate with actin, resulting in unopposed fusion.

Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Tomography

Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm, focusing on the actin cytoskeleton of Dictyostelium cells.

A Vesicular Transport Pathway Shuttles Cargo from Mitochondria to Lysosomes