When less is more: a more efficient vapour-diffusion protocol.

@article{Dunlop2003WhenLI,
  title={When less is more: a more efficient vapour-diffusion protocol.},
  author={Kirsty V Dunlop and Bart Hazes},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2003},
  volume={59 Pt 10},
  pages={
          1797-800
        }
}
Reducing protein consumption during crystallization screening is of utmost importance to crystallographers because of the time, effort and money that goes into producing pure protein. One approach is to reduce sample volumes with robotics, but a patent and the high cost of equipment limits access. Here, it is shown that the same result can be obtained by reducing the sample concentration in a modified vapour-diffusion protocol, the dilution method. In this protocol, the protein and mother… CONTINUE READING

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