Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes

  title={Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes},
  author={F. Hamel and M. Grondin and F. Denizeau and D. Averill-Bates and F. Sarhan},
  journal={Biotechnology and Bioengineering},
Hepatocytes are an important physiological model for evaluation of metabolic and biological effects of xenobiotics. They do not proliferate in culture and are extremely sensitive to damage during freezing and thawing, even after the addition of classical cryoprotectants. Thus improved cryopreservation techniques are needed to reduce cell injury and functional impairment. Here, we describe a new and efficient cryopreservation method, which permits long‐term storage and recovery of large… Expand
25 Citations
Wheat proteins improve cryopreservation of rat hepatocytes
  • 15
  • PDF
Wheat enolase demonstrates potential as a non-toxic cryopreservation agent for liver and pancreatic cells.
  • 6
  • PDF
In vitro cultivation and cryopreservation of duck embryonic hepatocytes.
  • 12
Hepatocyte cryopreservation: is it time to change the strategy?
  • 95
  • PDF
Inhibition of ice recrystallization and cryoprotective activity of wheat proteins in liver and pancreatic cells
  • 3
Metabolic Activity of Cytochrome P450 Isoforms in Hepatocytes Cryopreserved with Wheat Protein Extract
  • 19
  • PDF
Cryopreservation of insulin-secreting INS832/13 cells using a wheat protein formulation.
  • 4


Studies of chemical toxicity to fresh and cryopreserved rat hepatocytes.
  • 12
Cryopreservation of rat and dog hepatocytes for studies of xenobiotic metabolism and activation.
  • 60
Induction of cytochrome-P450 in cryopreserved rat and human hepatocytes.
  • 91
Survival and function of isolated hepatocytes after cryopreservation.
  • 82
Cultures with cryopreserved hepatocytes: applicability for studies of enzyme induction.
  • 83
Effect of cryopreservation on cytochrome P-450 enzyme induction in cultured rat hepatocytes.
  • 90
  • PDF
Optimization of cryoprotectants for cryopreservation of rat hepatocyte
  • 33
Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel.
  • 153
Preservation of the rate and profile of xenobiotic metabolism in rat hepatocytes stored in liquid nitrogen.
  • 57