Wheat dwarf virus Ac/Ds vectors: expression and excision of transposable elements introduced into various cereals by a viral replicon.

@article{Laufs1990WheatDV,
  title={Wheat dwarf virus Ac/Ds vectors: expression and excision of transposable elements introduced into various cereals by a viral replicon.},
  author={Juergen Laufs and Uwe Wirtz and Matthias Kammann and V Matzeit and Sabine Schaefer and J. L. Schell and A. P. Czernilofsky and Barbara Baker and Bruno Gronenborn},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 19},
  pages={7752-6}
}
The maize transposon Activator (Ac) and in vitro-generated nonautonomous derivatives thereof [Ac delta or Dissociation (Ds) elements] were inserted into the genome of a geminivirus of graminaceous plants, wheat dwarf virus, at a site that does not interfere with viral replication. These recombinant viral genomes were introduced into wheat, maize, and rice protoplasts, where rapid and efficient excision of Ac was observed. Excision was detected only in vectors in which, after transfection, the… CONTINUE READING

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