Wheat Germ Cell‐Free Expression System for Protein Production
@article{Vinarov2006WheatGC, title={Wheat Germ Cell‐Free Expression System for Protein Production}, author={Dmitriy A. Vinarov and Carrie L. Loushin Newman and Ejan M. Tyler and John L. Markley and Mark N. Shahan}, journal={Current Protocols in Protein Science}, year={2006}, volume={44} }
The Center for Eukaryotic Structural Genomics, in cooperation with Ehime University and CellFree Sciences, has developed a novel wheat germ cell‐free technology for the production of eukaryotic proteins. Protein production and purification are robust and scalable for high‐throughput applications. The protocols have been used to express and purify proteins from Arabidopsis thaliana, human, mouse, rat and zebra fish. This unit describes expression and purification protocols for both small‐scale…
22 Citations
Wheat germ cell‐free platform for eukaryotic protein production
- BiologyThe FEBS journal
- 2006
A platform that utilizes wheat germ cell‐free technology to produce protein samples for NMR structure determinations is described and their application to targets chosen from a variety of eukaryotic genomes is described.
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The integration of isotopically labeled proteins into the sequence of experiments required for NMR structure determination is outlined, and additional protocols for production of integral membrane proteins in the presence of either detergents or unilamellar liposomes are presented.
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The tremendous potential of the rapidly evolving and highly versatile CFPS systems are highlighted, making them more widely used as common tools to recombinantly prepare particularly challenging recombinant eukaryotic proteins.
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This work compares the uses of different cell-free translation systems and reviews recent findings that support the possibility of scaling up and thereby enhancing the industrial application of wheat germ cell free protein expression.
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Vectors designed for protein production in Escherichia coli and by wheat germ cell-free translation were tested using 21 well-characterized eukaryotic proteins chosen to serve as controls within the…
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The Center for Eukaryotic Structural Genomics
- BiologyJournal of Structural and Functional Genomics
- 2008
The technological innovations that underlie CESG’s platforms for bioinformatics and laboratory information management, target selection, protein production, and structure determination by X-ray crystallography or NMR spectroscopy are described.
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- 2012
BTEs confer the highest levels of translation of uncapped mRNAs in vitro and in vivo, while the capped omega sequence is most efficient in tobacco cells.
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