Wheat Germ Cell‐Free Expression System for Protein Production

@article{Vinarov2006WheatGC,
  title={Wheat Germ Cell‐Free Expression System for Protein Production},
  author={Dmitriy A. Vinarov and Carrie L. Loushin Newman and Ejan M. Tyler and John L. Markley and Mark N. Shahan},
  journal={Current Protocols in Protein Science},
  year={2006},
  volume={44}
}
The Center for Eukaryotic Structural Genomics, in cooperation with Ehime University and CellFree Sciences, has developed a novel wheat germ cell‐free technology for the production of eukaryotic proteins. Protein production and purification are robust and scalable for high‐throughput applications. The protocols have been used to express and purify proteins from Arabidopsis thaliana, human, mouse, rat and zebra fish. This unit describes expression and purification protocols for both small‐scale… 

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