What does the commonly used DCF test for oxidative stress really show?

  title={What does the commonly used DCF test for oxidative stress really show?},
  author={Markus Karlsson and Tino Kurz and Ulf T. Brunk and Sven Erik G. Nilsson and Christina I Frennesson},
  journal={The Biochemical journal},
  volume={428 2},
H(2)DCF-DA (dihydrodichlorofluorescein diacetate) is widely used to evaluate 'cellular oxidative stress'. After passing through the plasma membrane, this lipophilic and non-fluorescent compound is de-esterified to a hydrophilic alcohol [H(2)DCF (dihydrodichlorofluorescein)] that may be oxidized to fluorescent DCF (2',7'-dichlorofluorescein) by a process usually considered to involve ROS (reactive oxygen species). It is, however, not always recognized that, being a hydrophilic molecule, H(2)DCF… 

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