Western blotting/Edman sequencing using PVDF membrane.


The western blotting/Edman sequencing technique using polyvinylidene difluoride (PVDF) membrane is one of the most popular technique for determination of primary structure. A protein sample is transferred from a SDS-polyacrylamide gel electrophoresis (PAGE) gel onto a PVDF membrane by electroblotting. The membrane carrying the protein is directly subjected… (More)
DOI: 10.1007/978-1-59745-542-8_18


Cite this paper

@article{Komatsu2009WesternBS, title={Western blotting/Edman sequencing using PVDF membrane.}, author={Setsuko Komatsu}, journal={Methods in molecular biology}, year={2009}, volume={536}, pages={163-71} }