Western blot evaluation of siRNA delivery by pH-responsive peptides.

  title={Western blot evaluation of siRNA delivery by pH-responsive peptides.},
  author={Wanling Liang and A James Mason and Jenny K W Lam},
  journal={Methods in molecular biology},
Gene silencing, via RNA interference (RNAi) technologies using small interfering RNA (siRNA), has been developed as an important tool for target identification and validation in drug discovery and has huge therapeutic potential. However, effective delivery into cells presents a major challenge to the use of siRNA. pH-responsive cell-penetrating peptides have attracted considerable attention in recent years as delivery vectors due to their ability to transport their cargos across the biological… CONTINUE READING