Western blot analysis of antibody responses to influenza virion proteins.

@article{Qiu1992WesternBA,
  title={Western blot analysis of antibody responses to influenza virion proteins.},
  author={Dongxu Qiu and Gregory A Tannock and Richard D. Barry and David C Jackson},
  journal={Immunology and cell biology},
  year={1992},
  volume={70 ( Pt 3)},
  pages={
          181-91
        }
}
An immunoblotting procedure was developed to detect antibody responses in mice and humans to influenza virion proteins. The technique was capable of detecting 1.5 micrograms of haemagglutinin (HA) on nitrocellulose strips at a 1:5000 dilution of a mouse serum with an initial haemagglutination inhibition titre of 20. The effects of the use of the blocking agent Tween-20 on virion proteins were also studied. The commonly used concentration of 0.05% (v/v) Tween-20, when included in blocking and… CONTINUE READING