Western Blotting

  title={Western Blotting},
  author={Biji Theyilamannil Kurien and Robert Hal Scofield},
  volume={38 4},
Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer. This review describes the various procedures that… CONTINUE READING
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