Weak protein-cationic co-ion interactions addressed by X-ray crystallography and mass spectrometry.

@article{Bnas2014WeakPC,
  title={Weak protein-cationic co-ion interactions addressed by X-ray crystallography and mass spectrometry.},
  author={Philippe B{\'e}nas and Nicolas Auzeil and Laurent Legrand and Franck Brachet and Anne Regazzetti and Madeleine Ri{\`e}s-Kautt},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2014},
  volume={70 Pt 8},
  pages={2217-31}
}
The adsorption of Rb(+), Cs(+), Mn(2+), Co(2+) and Yb(3+) onto the positively charged hen egg-white lysozyme (HEWL) has been investigated by solving 13 X-ray structures of HEWL crystallized with their chlorides and by applying electrospray ionization mass spectrometry (ESI-MS) first to dissolved protein crystals and then to the protein in buffered salt solutions. The number of bound cations follows the order Cs(+) < Mn(2+) ≃ Co(2+) < Yb(3+) at 293 K. HEWL binds less Rb(+) (qtot = 0.7) than Cs… CONTINUE READING

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