Weak lignin-binding enzymes: a novel approach to improve activity of cellulases for hydrolysis of lignocellulosics.

@article{Berlin2005WeakLE,
  title={Weak lignin-binding enzymes: a novel approach to improve activity of cellulases for hydrolysis of lignocellulosics.},
  author={A. G. Berlin and Neil R. Gilkes and Arwa Kurabi and Renata Bura and Maobing Tu and Douglas G. Kilburn and John N. Saddler},
  journal={Applied biochemistry and biotechnology},
  year={2005},
  volume={121-124},
  pages={163-70}
}
Economic barriers preventing commercialization of lignocellulose-to-ethanol bioconversion processes include the high cost of hydrolytic enzymes. One strategy for cost reduction is to improve the specific activities of cellulases by genetic engineering. However, screening for improved activity typically uses "ideal" cellulosic substrates, and results are not necessarily applicable to more realistic substrates such as pretreated hardwoods and softwoods. For lignocellulosic substrates… CONTINUE READING

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