Weak anion exchange chromatographic profiling of glycoprotein isoforms on a polymer monolithic capillary.

@article{Liu2012WeakAE,
  title={Weak anion exchange chromatographic profiling of glycoprotein isoforms on a polymer monolithic capillary.},
  author={Jing Liu and Lianbing Ren and Yunchun Liu and Hengye Li and Zhen Liu},
  journal={Journal of chromatography. A},
  year={2012},
  volume={1228},
  pages={276-82}
}
High resolution separation of intact glycoproteins, which is essential for many aspects such as finger-print profiling, represents a great challenge because one glycoprotein can exhibit many isoforms with close physicochemical properties. Monolithic columns are important separation media for the separation of intact proteins due to its significant advantages such as easy preparation, high column efficiency and high permeability. However, there are few reports on high resolution profiling of… CONTINUE READING